Direct demonstration that ATP is in contact with Cys-137 in chaperonin GroEL.
نویسندگان
چکیده
The nonhydrolyzable ATP analogue ATP gamma S (adenosine 5'-3-O-(thio)triphosphate) is affinity cross-linked to GroEL by formation of a disulfide bridge in a peroxide-promoted reaction. By replacing with serine each of 3 cysteine residues in GroEL, it is shown that ATP gamma S specifically cross-links to Cys-137. It is thus demonstrated that the ATP bound to GroEL is in direct contact with Cys-137.
منابع مشابه
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 269 1 شماره
صفحات -
تاریخ انتشار 1994